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Testing the repatriated for SARS-Cov2: Should laboratory-based quarantine replace traditional quarantine?

Identifieur interne : 000180 ( 2020/Analysis ); précédent : 000179; suivant : 000181

Testing the repatriated for SARS-Cov2: Should laboratory-based quarantine replace traditional quarantine?

Auteurs : Jean Christophe Lagier [France] ; Philippe Colson [France] ; Hervé Tissot Dupont [France] ; Jérôme Salomon [France] ; Barbara Doudier [France] ; Camille Aubry [France] ; Frédérique Gouriet [France] ; Sophie Baron [France] ; Pierre Dudouet [France] ; Rémi Flores [France] ; Lucie Ailhaud [France] ; Philippe Gautret [France] ; Philippe Parola [France] ; Bernard La Scola [France] ; Didier Raoult [France] ; Philippe Brouqui [France]

Source :

RBID : PMC:7102645

Abstract

Background

An ongoing epidemic of respiratory diseases caused by a novel coronavirus (COVID 2019, SARS-CoV2) started in Wuhan, Hubei, in China at the end of December 2019. The French government decided to repatriate the 337 French nationals living in Wuhan and place them in quarantine in their home country. We decided to test them all for SARS-Cov2 twice in order to reduce anxiety among the population and decision-makers.

Methods

We investigated the presence of SARS-CoV-19 in asymptomatic carriers by testing all repatriated patients within the first 24 h of their arrival in France and at day 5. Viral RNA was extracted from pooled nasal and oropharyngeal swab fluids or sputum in the absence of nasal/oropharyngeal swabs. Detection of SARS-CoV-2 RNA was then carried out using several real-time reverse transcription (RT)-PCR assays.

Results

We tested 337 passengers at day 0 and day 5. All the tests for SARS-CoV2 were negative. By optimising the sampling process, sending samples sequentially and reducing the time-scale for biological analysis, we were able to test the samples within 5 h (including sampling, shipment and biological tests).

Conclusion

Optimising our procedures reduces anxiety and reassures the population and decision makers.


Url:
DOI: 10.1016/j.tmaid.2020.101624
PubMed: 32179125
PubMed Central: 7102645


Affiliations:


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PMC:7102645

Le document en format XML

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<name sortKey="Flores, Remi" sort="Flores, Remi" uniqKey="Flores R" first="Rémi" last="Flores">Rémi Flores</name>
<affiliation wicri:level="3">
<nlm:aff id="aff1">Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille</wicri:regionArea>
<placeName>
<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Ailhaud, Lucie" sort="Ailhaud, Lucie" uniqKey="Ailhaud L" first="Lucie" last="Ailhaud">Lucie Ailhaud</name>
<affiliation wicri:level="3">
<nlm:aff id="aff1">Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille</wicri:regionArea>
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<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Gautret, Philippe" sort="Gautret, Philippe" uniqKey="Gautret P" first="Philippe" last="Gautret">Philippe Gautret</name>
<affiliation wicri:level="3">
<nlm:aff id="aff3">Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille</wicri:regionArea>
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</placeName>
</affiliation>
</author>
<author>
<name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name>
<affiliation wicri:level="3">
<nlm:aff id="aff3">Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, IHU-Méditerranée Infection, Marseille</wicri:regionArea>
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<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="La Scola, Bernard" sort="La Scola, Bernard" uniqKey="La Scola B" first="Bernard" last="La Scola">Bernard La Scola</name>
<affiliation wicri:level="3">
<nlm:aff id="aff1">Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille</wicri:regionArea>
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<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name>
<affiliation wicri:level="3">
<nlm:aff id="aff1">Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille</wicri:regionArea>
<placeName>
<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Brouqui, Philippe" sort="Brouqui, Philippe" uniqKey="Brouqui P" first="Philippe" last="Brouqui">Philippe Brouqui</name>
<affiliation wicri:level="3">
<nlm:aff id="aff1">Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille, France</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea>Aix Marseille Univ, IRD, AP-HM, MEPHI, IHU Méditerranée Infection, Marseille</wicri:regionArea>
<placeName>
<region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Marseille</settlement>
</placeName>
</affiliation>
</author>
</analytic>
<series>
<title level="j">Travel Medicine and Infectious Disease</title>
<idno type="ISSN">1477-8939</idno>
<idno type="eISSN">1873-0442</idno>
<imprint>
<date when="2020">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass></textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<sec>
<title>Background</title>
<p>An ongoing epidemic of respiratory diseases caused by a novel coronavirus (COVID 2019, SARS-CoV2) started in Wuhan, Hubei, in China at the end of December 2019. The French government decided to repatriate the 337 French nationals living in Wuhan and place them in quarantine in their home country. We decided to test them all for SARS-Cov2 twice in order to reduce anxiety among the population and decision-makers.</p>
</sec>
<sec>
<title>Methods</title>
<p>We investigated the presence of SARS-CoV-19 in asymptomatic carriers by testing all repatriated patients within the first 24 h of their arrival in France and at day 5. Viral RNA was extracted from pooled nasal and oropharyngeal swab fluids or sputum in the absence of nasal/oropharyngeal swabs. Detection of SARS-CoV-2 RNA was then carried out using several real-time reverse transcription (RT)-PCR assays.</p>
</sec>
<sec>
<title>Results</title>
<p>We tested 337 passengers at day 0 and day 5. All the tests for SARS-CoV2 were negative. By optimising the sampling process, sending samples sequentially and reducing the time-scale for biological analysis, we were able to test the samples within 5 h (including sampling, shipment and biological tests).</p>
</sec>
<sec>
<title>Conclusion</title>
<p>Optimising our procedures reduces anxiety and reassures the population and decision makers.</p>
</sec>
</div>
</front>
<back>
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</back>
</TEI>
<affiliations>
<list>
<country>
<li>France</li>
</country>
<region>
<li>Provence-Alpes-Côte d'Azur</li>
<li>Île-de-France</li>
</region>
<settlement>
<li>Marseille</li>
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</list>
<tree>
<country name="France">
<region name="Provence-Alpes-Côte d'Azur">
<name sortKey="Lagier, Jean Christophe" sort="Lagier, Jean Christophe" uniqKey="Lagier J" first="Jean Christophe" last="Lagier">Jean Christophe Lagier</name>
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<name sortKey="Ailhaud, Lucie" sort="Ailhaud, Lucie" uniqKey="Ailhaud L" first="Lucie" last="Ailhaud">Lucie Ailhaud</name>
<name sortKey="Aubry, Camille" sort="Aubry, Camille" uniqKey="Aubry C" first="Camille" last="Aubry">Camille Aubry</name>
<name sortKey="Baron, Sophie" sort="Baron, Sophie" uniqKey="Baron S" first="Sophie" last="Baron">Sophie Baron</name>
<name sortKey="Brouqui, Philippe" sort="Brouqui, Philippe" uniqKey="Brouqui P" first="Philippe" last="Brouqui">Philippe Brouqui</name>
<name sortKey="Colson, Philippe" sort="Colson, Philippe" uniqKey="Colson P" first="Philippe" last="Colson">Philippe Colson</name>
<name sortKey="Doudier, Barbara" sort="Doudier, Barbara" uniqKey="Doudier B" first="Barbara" last="Doudier">Barbara Doudier</name>
<name sortKey="Dudouet, Pierre" sort="Dudouet, Pierre" uniqKey="Dudouet P" first="Pierre" last="Dudouet">Pierre Dudouet</name>
<name sortKey="Flores, Remi" sort="Flores, Remi" uniqKey="Flores R" first="Rémi" last="Flores">Rémi Flores</name>
<name sortKey="Gautret, Philippe" sort="Gautret, Philippe" uniqKey="Gautret P" first="Philippe" last="Gautret">Philippe Gautret</name>
<name sortKey="Gouriet, Frederique" sort="Gouriet, Frederique" uniqKey="Gouriet F" first="Frédérique" last="Gouriet">Frédérique Gouriet</name>
<name sortKey="La Scola, Bernard" sort="La Scola, Bernard" uniqKey="La Scola B" first="Bernard" last="La Scola">Bernard La Scola</name>
<name sortKey="Parola, Philippe" sort="Parola, Philippe" uniqKey="Parola P" first="Philippe" last="Parola">Philippe Parola</name>
<name sortKey="Raoult, Didier" sort="Raoult, Didier" uniqKey="Raoult D" first="Didier" last="Raoult">Didier Raoult</name>
<name sortKey="Salomon, Jerome" sort="Salomon, Jerome" uniqKey="Salomon J" first="Jérôme" last="Salomon">Jérôme Salomon</name>
<name sortKey="Tissot Dupont, Herve" sort="Tissot Dupont, Herve" uniqKey="Tissot Dupont H" first="Hervé" last="Tissot Dupont">Hervé Tissot Dupont</name>
</country>
</tree>
</affiliations>
</record>

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